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orthogonal image stacks  (Oxford Instruments)


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  • 99

    Structured Review

    Oxford Instruments orthogonal image stacks
    Orthogonal Image Stacks, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44474 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/orthogonal+stack/Imaris/pm38553447-173-0-6
    Average 99 stars, based on 44474 article reviews
    orthogonal image stacks - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Software:

    Article Title: Peripheral tissues reprogram CD8 + T cells for pathogenicity during graft-versus-host disease
    Article Snippet: .. Raw image data were processed with Fiji (ImageJ): drift was corrected with the Fiji plugin “Correct 3D drift” using the SHG channel as reference; a median filter (0.3 pixel) was applied to reduce background noise and autofluorescence was removed using the command Image Calculator to subtract crossover signal between channels: corrected channel 1 = channel 1 – channel 2 – channel 3 corrected channel 2 = channel 2 – channel 1 – channel 3 corrected channel 3 = channel 3 – channel 1 – channel 2 Compilation images (z-stack projection and yz orthogonal view) were created with Imaris 8.3 software (Bitplane). s6 Immunofluorescence staining of epidermal sheets Epidermal sheets were fixed in ice-cold acetone, blocked in a 0.25% fish gelatin, 10% goat serum solution and stained for LC with CD207 (eBioL31) (eBioscience, USA). .. Images were acquired with the Leica DMI4000b fluorescence microscope (Leica Microsystems, UK), using the Leica Application Suite Advanced Fluorescence v3.2 software (Leica Microsystems, UK).

    Immunofluorescence:

    Article Title: Peripheral tissues reprogram CD8 + T cells for pathogenicity during graft-versus-host disease
    Article Snippet: .. Raw image data were processed with Fiji (ImageJ): drift was corrected with the Fiji plugin “Correct 3D drift” using the SHG channel as reference; a median filter (0.3 pixel) was applied to reduce background noise and autofluorescence was removed using the command Image Calculator to subtract crossover signal between channels: corrected channel 1 = channel 1 – channel 2 – channel 3 corrected channel 2 = channel 2 – channel 1 – channel 3 corrected channel 3 = channel 3 – channel 1 – channel 2 Compilation images (z-stack projection and yz orthogonal view) were created with Imaris 8.3 software (Bitplane). s6 Immunofluorescence staining of epidermal sheets Epidermal sheets were fixed in ice-cold acetone, blocked in a 0.25% fish gelatin, 10% goat serum solution and stained for LC with CD207 (eBioL31) (eBioscience, USA). .. Images were acquired with the Leica DMI4000b fluorescence microscope (Leica Microsystems, UK), using the Leica Application Suite Advanced Fluorescence v3.2 software (Leica Microsystems, UK).

    Staining:

    Article Title: Peripheral tissues reprogram CD8 + T cells for pathogenicity during graft-versus-host disease
    Article Snippet: .. Raw image data were processed with Fiji (ImageJ): drift was corrected with the Fiji plugin “Correct 3D drift” using the SHG channel as reference; a median filter (0.3 pixel) was applied to reduce background noise and autofluorescence was removed using the command Image Calculator to subtract crossover signal between channels: corrected channel 1 = channel 1 – channel 2 – channel 3 corrected channel 2 = channel 2 – channel 1 – channel 3 corrected channel 3 = channel 3 – channel 1 – channel 2 Compilation images (z-stack projection and yz orthogonal view) were created with Imaris 8.3 software (Bitplane). s6 Immunofluorescence staining of epidermal sheets Epidermal sheets were fixed in ice-cold acetone, blocked in a 0.25% fish gelatin, 10% goat serum solution and stained for LC with CD207 (eBioL31) (eBioscience, USA). .. Images were acquired with the Leica DMI4000b fluorescence microscope (Leica Microsystems, UK), using the Leica Application Suite Advanced Fluorescence v3.2 software (Leica Microsystems, UK).



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